PB0562 - Effects of high-fat and high-carbohydrate meals on coagulation and anticoagulation factors and lipid profile

Background: Lipid profile is affected by food intake and nonfasting as well as fasting triglycerides (TG) levels are associated with increased risk of cardiovascular disease. We found a positive correlation of fasting plasma protein S (PS) levels with apoC-Ⅱ, a key-regulator of TG hydrolysis.

Aims: To examine the effects of consumption of a high-fat (HF) and a high-carbohydrate (HC) meal on coagulation and anticoagulation factors and lipid profile.

Methods: In a randomized cross-over study, 12 healthy Japanese nonobese young women (21.3±0.5 years) consumed isocaloric meals consisting predominantly of either fat or carbohydrate: The meals were served on two separate days (7-11 days apart). Hemostatic and lipid parameters were determined in blood collected before (fasting 12h) and after (2.5 and 5.5 h) eating a meal. To test for differences across time, a one-way repeated measures ANOVA followed by multiple comparison was used. The study was approved by the local ethics committee and all participants gave written informed consent.

Results: Consumption of HF meal led to significant increases in TG and small dense LDL cholesterol and decreases in LDL cholesterol, HDL cholesterol, apoA-I, apoB, apo C-Ⅱ, apo C-Ⅲ and apoE. After consumption of HC meal, all these parameters except for apo C-Ⅱ decreased. Insulin levels increased particularly after HC meal. Total PS antigen levels were not changed postprandially. Factor Ⅶ:C was significantly increased after consumption of HF meal but was decreased after HC meal. Free PS antigen, protein C antigen, fibrinogen levels decreased only after consumption of HC meal.

Conclusion(s): We confirmed increased Ⅶ:C during postprandial triglyceridemia after consumption of HF meal, suggesting a presence of hypercoagulable state. In contrast, Ⅶ:C, free PS antigen, PC antigen and fibrinogen as well as most lipoprotein components were decreased after consumption of HC meal. Total PS antigen levels were not affected by food intake.