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    Poster Networking

    Coagulation and Natural Anticoagulants

    Poster Networking Session

    PB0527 - Assessment of the rats model of hemophilic arthritis by Micro CT scanning

    Monday, June 26, 2023
    18:30 – 19:30 ET
    Room: Exhibition

      Co-Author(s)

    • Shuang Chen, master of medicine

      North China University of Technology Affiliated Hospital
      Tangshan, Hebei, China (People's Republic)

      • Co-Author(s)

    • SZ

      Shi-Bo Zhang

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • YX

      Yan-Yan Xie

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • JX

      Ji-Ji Xu

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • JY

      Jun Yang

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • WW

      Wen-Qi Wu

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • LW

      Lin-Hong Wang

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    • DL

      Dong-Nan Li

      physician
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

      • Presenting Author(s)

    • ZY

      Zhen-Yu Yan

      Professor
      Affiliated Hospital of North China University of Technology
      Tangshan, Hebei, China (People's Republic)

    Background: The incidence rate of hemophilic arthropathy is increasing but mechanisms are not completely clear. Therefore, it is particularly important to transform hemophilic arthropathy into an animal model for pathophysiological research. Micro CT reconstruction can form high-resolution 3D images, which is convenient to observe the pathological process of bone and joint related diseases.

    Aims: To establish rat models of hemophilic arthritis with different severity and assess with Micro CT.

    Methods: The model of hemophilic arthritis was induced by acupuncture of the patellar ligament of the knee joint of hemophilic rats. The model group was randomly divided into acupuncture 2 times group (day 0, 14), acupuncture 3 times group (day 0, 14, 28), acupuncture 4 times group (day 0, 14, 28, 42), acupuncture bilateral patellar ligaments, and euthanized at day 30. The construction effects of different groups of joint models were evaluated by Micro-CT and histomorphology staining.

    Results: Micro-CT three-dimensional reconstruction analysis, the Tb.BMD, Tb.BV/TV, Tb.Th of the cartilage under the tibial plateau in the acupuncture groups 2, 3 and 4 were significantly lower than those in the normal group (P < 0.001). The 4 times acupuncture group was significantly lower than the 2 and 3 groups (P < 0.001). And the 3 times acupuncture group was significantly lower than the 2 groups (P < 0.001). Histomorphology staining observation showed that the number and morphology of chondrocytes in the acupuncture twice group changed slightly. The three times of acupuncture group was slightly worse. However, the four times of acupuncture group observed a sharp decrease in chondrocytes and disorder in morphology, accompanied by rough fracture of cartilage surface.

    Conclusion(s): Acupuncture of the patellar ligament of the knee joint of hemophilic rats for 2, 3 and 4 times can build a rat model of increasingly severe hemophilic arthritis.Micro CT is conducive to the study of the pathological progress of hemophilic arthropathy.