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    Poster Networking

    Venous Thromboembolism

    Poster Networking Session

    PB1022 - MicroRNAs expression before and after exercise in patients with residual thrombosis after pulmonary embolism.

    Monday, June 26, 2023
    18:30 – 19:30 ET
    Room: Exhibition

      Co-Author(s)

    • VS

      Verónica Sánchez-López, PhD

      Postdoctoral Researcher
      Instituto de Biomedicina de Sevilla (IBiS). Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
      Seville, Andalucia, Spain

    • BB

      Berenice Brown Arreola

      Pulmologist
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

    • DH

      David Hervás Marín

      Professor
      Departamento de Estadística e Investigación Operativa Aplicadas y Calidad. Universitat Politècnica de València, Valencia, Spain
      Valencia, Comunidad Valenciana, Spain

    • NS

      Noelia Sola Idígora

      Biology degree
      Instituto de Biomedicina de Sevilla (IBiS). Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
      Seville, Andalucia, Spain

    • EJ

      Ezequiel Juan Saborido

      Laboratory technician
      Instituto de Biomedicina de Sevilla (IBiS). Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
      Seville, Andalucia, Spain

    • MN

      María Dolores Nuñez Ollero

      Nurse
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

    • MR

      María Rosario Ruiz Serrano De La Espada

      Nurse
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

    • CF

      Cándido Fernández García

      Nurse
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

    • TE

      Teresa Elías Hernandez

      Pulmonologist
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

    • LJ

      Luis Jara-Palomares

      Senior consultant
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Sevilla, Andalucia, Spain

    • Julia Oto

      Postdoctoral Researcher
      Hemostasis, Thrombosis, Arteriosclerosis and Vascular Biology Research Group, Medical Research Institute Hospital La Fe, Valencia, Spain
      Valencia, Comunidad Valenciana, Spain

    • PM

      Pilar Medina

      PI
      Hemostasis, Thrombosis, Arteriosclerosis and Vascular Biology Research Group, Medical Research Institute Hospital La Fe, Valencia, Spain
      Valencia, Comunidad Valenciana, Spain

    • PC

      Pilar Cejudo Ramos

      Physical medicine rehabilitation doctor
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Seville, Andalucia, Spain

      • Presenting Author(s)

    • Remedios Otero-Candelera, Doctor (she/her/hers)

      Ph.D., M.D. Pulmonologist
      Hospital Universitario Virgen del Rocío, Seville, Spain
      Sevilla, Andalucia, Spain

    Background: Persistent lung perfusion defects after pulmonary embolism (PE) are due to residual thrombosis, the key of severe sequalae as chronic thromboembolic pulmonary hypertension (CTEPH). Physical activity is useful on cardiovascular diseases recover.

    Aims: To study microRNAs (miRNAs) dysregulated before and after acute exercise in patients with persistent lung perfusion defects after a PE. It is part of a broader study that evaluate the exercise training effect on lung perfusion evolution after PE [ClinicalTrials.gov Identifier: NCT04150003]

    Methods: Patients with persistent perfusion defects after one month follow-up of PE were recruited. Exercise responsiveness was assessed using cardiopulmonary exercise testing (CPET). Patient blood samples were drawn before and after exercise. Plasma RNA was extracted and 179 miRNAs expression was quantified. Logistic regression model and mixed linear model were employed to data analysis. Selection of miRNAs was performed based on maximum probability of effect (MPE). Mirwalk platform was used to miRNAs in silico analysis.

    Results: Fifteen patients (52.48 ±16 years) were analyzed, 53.3% were women. Results highlighted two miRNAs with higher MPE: miR-16-5p and miR-451a (MPE: 78.12 % and 84.23% respectively (Figure 1). Regarding correlation, a negative association for miR-16-5p and miR-451a and peak oxygen consumption VO2 (peak VO2, mL/kg/min) was observed (p < 0.001 and p = 0.009 respectively, Figure 2). Regarding ventilatory equivalent of CO2 at the anaerobic threshold (VE/VCO2 at AT) and selected miRNAs, it was found a positive association between VE/VCO2 at AT and increased miR-16-5p and miR-451a expression (p < 0.001 and p = 0.009 respectively, Figure 2). In silico analysis, we observed miR-16-5p regulates expression of genes implicated in cell cycle, hypoxia or vascular endothelial growth factor pathways.

    Conclusion(s): MicroRNAs expression would be associated with responsiveness to exercise in patients with residual thrombosis after PE. These miRNAs might act by regulating pathways involved in CTEPH development.

    Funding: SEPAR (PI22/1334), ISCIII-FIS (PI18//01640), CSYF-RH-0116-2020.