PB0754 - Neutrophil extracellular traps as a marker of procoagulant state in oncogynecological patients

Monday, June 26, 2023

18:30 – 19:30 ET

Room: Exhibition

Co-Author(s)

Ekaterina Slukhanchuk, PhD

Professor of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia

Co-Author(s)

VB

Victoria Bitsadze

Professor of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia
Jamilya Khizroeva, MD

Professor of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia
AS

Antonina Solopova

Professor of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia
Jean-Christophe R. Gris, MD, PhD

Professor
Department of Hematology, CHU Nîmes, Univ Montpellier, Nîmes, France
Nimes, Languedoc-Roussillon, France
Nilufar Gashimova, Postgraduate

Postgraduate student of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia
YP

Yuliya Popenova

Doctor
City Clinical Hospital 67 named after L.A. Vorokhobov of the Department of Health of the City of Moscow
Москва, Moskva, Russia

Presenting Author(s)

AM

Alexander Makatsariya

Head of Obstetrics, gynecology and perinatal medicine Department
I.M.Sechenov First Moscow State Medical University
Moscow, Moskva, Russia

Background: Hemostasis disorders and thrombosis in cancer patients is one of the leading causes of mortality. Recent studies have shown that neutrophil extracellular traps have procoagulant and proinflammatory potential, initiate thrombosis and participate in its progression.

Aims: Evaluation of the procoagulant activity of NETs in oncogynecological patients.

Methods: A study was conducted with the participation of 120 patients aged 32 to 72 years who were hospitalized in a hospital for planned surgical treatment or chemotherapy. The main group included 87 patients with malignant neoplasms of the female genital organs and mammary glands: uterine body cancer (n = 18), ovarian cancer (n = 26), cervical cancer – cervical adenocarcinoma (n = 13), breast cancer (n = 30). The control group consisted of 33 healthy women. Concentrations of citrullinated histone H3 (citH3), myeloperoxidase antigen (MRO:Ag), D-dimer and concentration of thrombin-antithrombin complexes in blood plasma were evaluated in all patients.

Results: There were no significant differences in the mean values of age and the number of neutrophils between the patients and the control group. In all groups, an increase in the concentration of netosis markers – citH3 and MPO:Ah (p < 0.0001) was statistically significant compared to the control group (Table 1). Correlation analysis showed a significant dependence of the increase in the concentration of thrombin antithrombin complexes (TAT) on the concentration of citH3 and MPO:Ag (Table 2). The revealed changes were more pronounced in patients with later stages of the process.

Conclusion(s): Tumor tissue creates conditions that stimulate neutrophils to release procoagulant extracellular traps, which, in turn, not only contribute to the creation of a powerful procoagulant state, but also are one of the factors that ensure metastasis. The development of a therapy aimed at NETs is potentially able to influence the hemostasis system in cancer patients, reduce the risk of thrombosis and the intensity of metastasis processes.