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    Poster Networking

    Vascular Biology

    Poster Networking Session

    PB1411 - Study of endothelial dysfunction in patients with unprovoked venous thromboembolism through endothelial colony-forming cell in vitro characterization

    Tuesday, June 27, 2023
    18:30 – 19:30 ET
    Room: Exhibition

      Co-Author(s)

    • AC

      Assunta Cancellara

      PhD Student
      Unit of Clinical and Experimental Immunology (UCEI) - Department of Medical Biotechnologies and Translational Medicine, University of Milan; IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    • MB

      Monica Bacci

      Biologist specialized in clinical biochemistry
      Center for Thrombosis and Hemorrhagic Diseases, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    • RC

      Roberta Ciceri

      Master Degree Student
      Unit of Clinical and Experimental Immunology (UCEI) - Department of Medical Biotechnologies and Translational Medicine, University of Milan; IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    • ER

      Erica Romualdi

      MD; PhD; Staff physician
      Centro Trombosi ed Emostasi, Ospedale di Circolo e Fondazione Macchi, ASST Sette Laghi, Varese, Italy
      Varese, Lombardia, Italy

    • VP

      Valentina Pessi

      MD, Resident
      Università dell'Insubria - Dipartimento di Medicina e Chirurgia, Varese, Italy
      Varese, Lombardia, Italy

    • FT

      Fabio Tumminello

      MD, Resident
      Center for Thrombosis and Hemorrhagic Diseases, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    • MF

      Martina Fantuzzi

      MD, Resident
      Università dell'Insubria - Dipartimento di Medicina e Chirurgia, Varese, Italy
      Varese, Lombardia, Italy

    • CL

      Corrado Lodigiani

      MD; PhD; Center Responsible
      Center for Thrombosis and Hemorrhagic Diseases, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    • MD

      Marco Paolo Donadini

      MD, Associate Professor
      Centro Trombosi ed Emostasi, Ospedale di Circolo e Fondazione Macchi, ASST Sette Laghi, Varese, Italy
      Varese, Lombardia, Italy

    • SD

      Silvia Della Bella

      MD, PhD, Associate Professor
      Unit of Clinical and Experimental Immunology (UCEI) - Department of Medical Biotechnologies and Translational Medicine, University of Milan, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

      • Presenting Author(s)

    • Francesca Calcaterra, PhD

      PhD, Staff Scientist
      Unit of Clinical and Experimental Immunology (UCEI) - Department of Medical Biotechnologies and Translational Medicine, University of Milan; IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

      • Co-Author(s)

    • DM

      Domenico Mavilio

      MD, PhD, Full Professor – Head of UCEI
      Unit of Clinical and Experimental Immunology (UCEI) - Department of Medical Biotechnologies and Translational Medicine, University of Milan, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
      Rozzano, Lombardia, Italy

    Background: Venous thromboembolism (VTE) often occurs in the presence of provoking factors - major ones induced provoked VTE (pVTE) whereas minor ones support weakly provoked (wp) VTE. Nevertheless, up to 50% of VTE cases occurs in absence of known triggering factors. Such events are defined as “unprovoked” (uVTE) and have a high risk of recurrence due to the unknown pathogenesis. Since endothelial dysfunction (ED) play a crucial role in triggering thrombus formation, possible presence of endothelial alterations deserves to be investigated in uVTE patients. In this regard, Endothelial colony-forming cells (ECFCs) represents a valuable tool to study patient-specific endothelium.

    Aims: To investigate the presence of alterations in VTE patient ECFCs thus identifying endothelium perturbation(s) involved in uVTE pathogenesis.

    Methods: ECFCs were obtained from VTE patients – classified as uVTE, wpVTE, or pVTE - and matched healthy donors (HDs). ECFC isolation efficiency and senescence were evaluated. ED was assessed by analyzing: platelet deposition and fibrin formation through thrombogenesis assay; thrombin formation through thrombin generation assay (TGA) on ECFCs; ECFC expression of the adhesion molecule CD106 and the pro-coagulant molecule Tissue Factor by flow cytometry.

    Results: ECFCs were isolated from all the three subgroups of VTE patients with a similar efficiency as HDs but showed a higher rate of early senescence (passage ≤3). In thrombogenesis assay, an increased platelet deposition was observed on uVTE ECFCs compared with HD ECFCs. Moreover, a reduction of the lag phase and an increase in thrombin formation (as Peak) was observed in TGA on uVTE ECFCs.

    Conclusion(s): Our results demonstrate that uVTE ECFCs are constitutively activated and that such ED is typical of uVTE thus supporting the ECFC use to investigate ED. Furthers studies are ongoing to disclose the pathways underlying the ED in uVTE and assess whether they are associated with uVTE only or are shared with secondary VTE.