PB0906 - Modified Platelet Storage Devices to Improve Quality During Storagee

Background: Platelet transfusion is a lifesaving therapy for trauma and various disorders. Current platelet storage devices limit their shelf life to 5-7 days, which diminishes the supply of these cells. The bio-incompatible storage bag materials activate platelets and lead to a decreased product quality in a process known as the Platelet Storage Lesion (PSL). Platelet concentrates are also prone to bacterial contamination which results in adverse transfusion reactions and sepsis. To combat these pathogens, pathogen inactivation technologies (PITs), a host of techniques which destroy nucleic acids, have recently been brought to market. Despite their effectiveness. PITs severely damage platelets and exacerbate the PSL. This project aims to develop a biocompatible storage device for platelets which extends the storage life of cells and prevents bacterial proliferation. Our group has developed a universal anti-biofilm coating based on polydopamine co-assembly with an assortment of ultra-large hydrophilic polymers which can be deposited on existing storage devices. This coating displays antibacterial properties and can decrease adverse platelet-bag interactions. Through this project, we hope to improve the shelf-life, quality, and supply of these lifesaving cells.

Aims: a) Extend the shelf-life of the platelets by reducing the damage they sustain from the storage devices. b) Trap platelet-activating groups present in the plasma to reduce their activation, thereby further reducing platelet activation. c) Optimize the coating for anti-microbial function.

Methods: Platelet activation measures:
Platelet activation and apoptosis during storage are assessed using surface expression of P-selectin and phosphatidylserine

Metabolomic quality:
Platelet metabolism is evaluated via pH, glucose, lactate, pO2, and pCO2.

Platelet functional analysis:
Platelet function is determined by rotational thromboelastometry (ROTEM) and aggregometry

Results: Our coating has successfully demonstrated a marked reduction in bacterial proliferation, and non-inferiority in platelet lifespan.

Conclusion(s): So far, our coating appears to work well for our anti-bacterial goals, and has demonstrated non-inferiority in terms of platelet storage.